P53 Rather Than Β-Catenin Mediated the Combined Hypoglycemic Effect of Innamomum Cassia (L.) and Zingiber of Ficinale Roscoe in The Streptozotocin-Induced Diabetic Model

Background
The antioxidant, hypoglycemic, and insulin-enhancing effects of ginger and cinnamon were previously
confirmed in experimental and human studies, while the combined effect of ginger and cinnamon was not thoroughly investigated until now.
Objectives
This study was designed to assess the antidiabetic effect of administration of ginger (Zingiber officinale Roscoe) and cinnamon (Cinnamomum cassia L.) in streptozotocin (STZ)-induced diabetic rats compared to metformin and to explain themechanism behind this effect.
Materials and methods: STZ was utilized to induce diabetes mellitus in male Sprague–Dawley rats.
Assessments of fasting blood glucose level (BGL), the total antioxidant capacity (TAC), serum insulin,
HOMA-IR, and HOMA–β cells were performed. Pancreatic gene expression of β-catenin and p53 was
assessed using RTPCR.
Assessment of histopathological alterations of pancreatic islet cells was performed using routine and
immunohistochemical techniques.
Results
BGL sig