Curcuma Longa for Protecting Chicks Against Newcastle Disease Virus Infection and Immunosuppressive Effect of Marek’s Disease Viral Vaccine

A total of 500 one day old Hubbard chicks fed balanced ration , reared in good hygienic measures and divided into 10 groups (n=50 /G). G1 (neither vaccinated nor treated), G2 vaccinated with NDV, G3 (vaccinated with MDV Rispen strain), G4 (vaccinated with MDV and NDV), G5 (vaccinated with MDV, treated with thymus extract) G6 (vaccinated with MDV, NDV, treated with thymus extract), G7 (vaccinated with MDV, treated with Nigella Sativa (NS), and G8 (vaccinated with MDV and NDV and treated with NS). Group 9 vaccinated with MDV, treated with Curcuma Longa, and G 10 vaccinated with MDV, NDV and treated with Curcuma Longa. Chicks recived NDV vaccine Hitciner B-1 at 7th day of age, boosted with LaSota strain at 21st day of age in drinking water. Groups received MDV S/C injection vaccine (0.2ml/chick) at one day of age. Sera of all chicks were collected at 10, 14, 17, 21, 28, 35, and 42 of age for determining the antibodies against NDV using HI test. Heparinized blood samples were collected at